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Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid

Received: 14 May 2013     Published: 10 June 2013
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Abstract

The peculiarities of changes of phenotype properties in MCF-7 human breast cancer cells with different sensitivity to cytostatic agents under the influence of nanoparticles of magnetic fluid were studied. It is defined that the biggest manifestations of magnetic fluid effect both by its essence and direction are observed in cells of resistant MCF-7/Dox line and parental MCF-7/S line in comparison with MCF-7/CP (cisplatin-resistant cell line). In particular, these are more apparent cytotoxic effects and ability to colony-formation, increase of adhesive properties, decrease of invasiveness and proliferative activity, changes of surface charge density and electrokinetic potential as well as lipid composition of cells. The defined differences between MCF-7 cells with different sensitivity to cytostatic agents according to phenotypic manifestations of correlations (magnetic fluid effect – reactive response) are evidence of the individual peculiarities of free radicals formation in cells and development of oxidative stress. The indicated phenomenon of selective reactive changes in cells by effect of magnetic fluid is necessary to take into account in development of systems of directed transport of antitumor drugs.

Published in Cancer Research Journal (Volume 1, Issue 1)
DOI 10.11648/j.crj.20130101.12
Page(s) 7-14
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

MCF-7 Cells, Nanoparticles of Magnetic Fluid, Drug Resistance, Cytotoxic Effects, Adhesion, Invasion, Proliferative Activity, Free Radical Processes, Oxidative Stress, Lipids

References
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  • APA Style

    Lukianova N Yu., Naleskina L A., Bezdenezhnykh N O., Kunskaya L M., Demash D V., et al. (2013). Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid. Cancer Research Journal, 1(1), 7-14. https://doi.org/10.11648/j.crj.20130101.12

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    ACS Style

    Lukianova N Yu.; Naleskina L A.; Bezdenezhnykh N O.; Kunskaya L M.; Demash D V., et al. Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid. Cancer Res. J. 2013, 1(1), 7-14. doi: 10.11648/j.crj.20130101.12

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    AMA Style

    Lukianova N Yu., Naleskina L A., Bezdenezhnykh N O., Kunskaya L M., Demash D V., et al. Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid. Cancer Res J. 2013;1(1):7-14. doi: 10.11648/j.crj.20130101.12

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  • @article{10.11648/j.crj.20130101.12,
      author = {Lukianova N Yu. and Naleskina L A. and Bezdenezhnykh N O. and Kunskaya L M. and Demash D V. and Yanish Yu V. and Todor I N. and Chekhun V F.},
      title = {Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid},
      journal = {Cancer Research Journal},
      volume = {1},
      number = {1},
      pages = {7-14},
      doi = {10.11648/j.crj.20130101.12},
      url = {https://doi.org/10.11648/j.crj.20130101.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20130101.12},
      abstract = {The peculiarities of changes of phenotype properties in MCF-7 human breast cancer cells with different sensitivity to cytostatic agents under the influence of nanoparticles of magnetic fluid were studied. It is defined that the biggest manifestations of magnetic fluid effect both by its essence and direction are observed in cells of resistant MCF-7/Dox line and parental MCF-7/S line in comparison with MCF-7/CP (cisplatin-resistant cell line). In particular, these are more apparent cytotoxic effects and ability to colony-formation, increase of adhesive properties, decrease of invasiveness and proliferative activity, changes of surface charge density and electrokinetic potential as well as lipid composition of cells. The defined differences between MCF-7 cells with different sensitivity to cytostatic agents according to phenotypic manifestations of correlations (magnetic fluid effect – reactive response) are evidence of the individual peculiarities of free radicals formation in cells and development of oxidative stress. The indicated phenomenon of selective reactive changes in cells by effect of magnetic fluid is necessary to take into account in development of systems of directed transport of antitumor drugs.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid
    AU  - Lukianova N Yu.
    AU  - Naleskina L A.
    AU  - Bezdenezhnykh N O.
    AU  - Kunskaya L M.
    AU  - Demash D V.
    AU  - Yanish Yu V.
    AU  - Todor I N.
    AU  - Chekhun V F.
    Y1  - 2013/06/10
    PY  - 2013
    N1  - https://doi.org/10.11648/j.crj.20130101.12
    DO  - 10.11648/j.crj.20130101.12
    T2  - Cancer Research Journal
    JF  - Cancer Research Journal
    JO  - Cancer Research Journal
    SP  - 7
    EP  - 14
    PB  - Science Publishing Group
    SN  - 2330-8214
    UR  - https://doi.org/10.11648/j.crj.20130101.12
    AB  - The peculiarities of changes of phenotype properties in MCF-7 human breast cancer cells with different sensitivity to cytostatic agents under the influence of nanoparticles of magnetic fluid were studied. It is defined that the biggest manifestations of magnetic fluid effect both by its essence and direction are observed in cells of resistant MCF-7/Dox line and parental MCF-7/S line in comparison with MCF-7/CP (cisplatin-resistant cell line). In particular, these are more apparent cytotoxic effects and ability to colony-formation, increase of adhesive properties, decrease of invasiveness and proliferative activity, changes of surface charge density and electrokinetic potential as well as lipid composition of cells. The defined differences between MCF-7 cells with different sensitivity to cytostatic agents according to phenotypic manifestations of correlations (magnetic fluid effect – reactive response) are evidence of the individual peculiarities of free radicals formation in cells and development of oxidative stress. The indicated phenomenon of selective reactive changes in cells by effect of magnetic fluid is necessary to take into account in development of systems of directed transport of antitumor drugs.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

  • Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

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