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Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation

Received: 24 November 2014     Accepted: 26 November 2014     Published: 27 December 2014
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Abstract

Microgravity simulation in rats resulted to changes in eye lavage content. Molecular subfraction analysis with laser correlation spectroscopy revealed significant differences in the pattern of particle distribution in five intervals: 1.9 -2.56 nm, 4.64-6.25 nm, 91.3 nm, 165-300 nm, and 400-734 nm. Microgravity modeling induced a series of interrelated processes in the retina of experimental rats manifesting in decreasing of total and layer-by-layer thickness of the retina, mild edema, and consequently, the increasing of specific density of neurons. In the inner nuclear layer, the cell density decreased because of the amacrine and bipolar cells. These changes could be explained by relative ischemia of the retinal layers, which are dependent on the retinal vessels circulation system.

Published in American Journal of Life Sciences (Volume 3, Issue 1-2)

This article belongs to the Special Issue Space Flight Factors: From Cell to Body

DOI 10.11648/j.ajls.s.2015030102.14
Page(s) 18-24
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Amacrine Cells, Bipolar Cells, Laser Correlation Spectroscopy, Microgravity Simulation, Muller Cells, Retinal Vessels

References
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[5] Roberts J.E., Kukielczak B.M., Chignell C.F., Sik B.H., Hu D.N., Principato M.A., “Simulated microgravity induced damage in human retinal pigment epithelial cells,” Mol Vis., vol.30(12), 2006, pp. 633-538.
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[9] Karganov M., Skalny A., Alchinova I., Khlebnikova N., Grabeklis A., Lakarova E., Eisazadeh S., “Combined use of laser correlation spectroscopy and ICP-AES, ICP-MS determination of macro- and trace elements in human biosubstrates for intoxication risk assessment,” Trace elements and electrolytes, vol. 28, No 2, 2011, pp.124-127.
[10] B. E. Crucian, R. P. Stowe, D. L. Pierson, C. F. Sams, “Immune system dysregulation following short- vs longduration spaceflight,” Aviation Space and Environmental Medicine, vol. 79, No 9, 2008, pp. 835–843.
[11] G. Sonnenfeld, J. S. Butel, W. T. Shearer, “Effects of the space flight environment on the immune system,” Reviews on Environmental Health, vol. 18, No. 1, 2003, pp. 1–17.
[12] Girardi C., De Pittà C., Casara S., Calura E., Romualdi C., Celotti L., Mognato M., “Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity,” BioMed Research International, 2014, Article ID 296747, 16 pages, http://dx.doi.org/10.1155/2014/296747
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Cite This Article
  • APA Style

    Margarita Vyalkina, Anatoly Fedorov, Erika Eskina, Yulia Medvedeva, Elena Arkhipova, et al. (2014). Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation. American Journal of Life Sciences, 3(1-2), 18-24. https://doi.org/10.11648/j.ajls.s.2015030102.14

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    ACS Style

    Margarita Vyalkina; Anatoly Fedorov; Erika Eskina; Yulia Medvedeva; Elena Arkhipova, et al. Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation. Am. J. Life Sci. 2014, 3(1-2), 18-24. doi: 10.11648/j.ajls.s.2015030102.14

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    AMA Style

    Margarita Vyalkina, Anatoly Fedorov, Erika Eskina, Yulia Medvedeva, Elena Arkhipova, et al. Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation. Am J Life Sci. 2014;3(1-2):18-24. doi: 10.11648/j.ajls.s.2015030102.14

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  • @article{10.11648/j.ajls.s.2015030102.14,
      author = {Margarita Vyalkina and Anatoly Fedorov and Erika Eskina and Yulia Medvedeva and Elena Arkhipova and Marina Lebedeva and Nadezhda Khlebnikova and Mikhail Baranov and Mikhail Karganov},
      title = {Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {1-2},
      pages = {18-24},
      doi = {10.11648/j.ajls.s.2015030102.14},
      url = {https://doi.org/10.11648/j.ajls.s.2015030102.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030102.14},
      abstract = {Microgravity simulation in rats resulted to changes in eye lavage content. Molecular subfraction analysis with laser correlation spectroscopy revealed significant differences in the pattern of particle distribution  in five intervals: 1.9 -2.56 nm, 4.64-6.25 nm, 91.3 nm, 165-300 nm, and 400-734 nm. Microgravity modeling induced a series of interrelated processes in the retina of experimental rats manifesting in decreasing of total and layer-by-layer thickness of the retina, mild edema, and consequently, the increasing of specific density of neurons.  In the inner nuclear layer, the cell density decreased because of the amacrine and bipolar cells. These changes could be explained by relative ischemia of the retinal layers, which are dependent on the retinal vessels circulation system.},
     year = {2014}
    }
    

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    T1  - Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation
    AU  - Margarita Vyalkina
    AU  - Anatoly Fedorov
    AU  - Erika Eskina
    AU  - Yulia Medvedeva
    AU  - Elena Arkhipova
    AU  - Marina Lebedeva
    AU  - Nadezhda Khlebnikova
    AU  - Mikhail Baranov
    AU  - Mikhail Karganov
    Y1  - 2014/12/27
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.s.2015030102.14
    DO  - 10.11648/j.ajls.s.2015030102.14
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 18
    EP  - 24
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2015030102.14
    AB  - Microgravity simulation in rats resulted to changes in eye lavage content. Molecular subfraction analysis with laser correlation spectroscopy revealed significant differences in the pattern of particle distribution  in five intervals: 1.9 -2.56 nm, 4.64-6.25 nm, 91.3 nm, 165-300 nm, and 400-734 nm. Microgravity modeling induced a series of interrelated processes in the retina of experimental rats manifesting in decreasing of total and layer-by-layer thickness of the retina, mild edema, and consequently, the increasing of specific density of neurons.  In the inner nuclear layer, the cell density decreased because of the amacrine and bipolar cells. These changes could be explained by relative ischemia of the retinal layers, which are dependent on the retinal vessels circulation system.
    VL  - 3
    IS  - 1-2
    ER  - 

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Author Information
  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of the Fundamental Investigations in Ophthalmology, Research Institute of Eye Diseases, Moscow, Russia

  • Ophthalmological Department of Federal Medical-Biology Agency of Russia, Sphere Eye Clinic, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Experimental Clinical Dept., Research Institute for Space Medicine, Federal Biomedical Agency of Russia, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

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