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Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook

Received: 16 June 2015     Accepted: 17 June 2015     Published: 15 July 2015
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Abstract

Background: mussaenda roxburghii hook. Belongs to family rubiaceae, is a perennial shrub moist area of valley and grows in the foothills. Objective: the present investigation was carried out to phytochemicals and pharmacological activity such anti-inflammatory, cardioprotective properties of methanolic crude extract & its fractions such as dcm extract (fraction 1), ethyl acetate extract (fraction2). Materials and methods: residual methanolic extract (fraction 3) of mussaenda roxburghii leaves which were obtained by solvent-solvent extraction process from crude methanolic extract of mussaenda roxburghii. Anti-inflammatory activity was measured by observing the mean inhibition of protein denaturation. Cardioprotective activity was evaluated by thrombolytic assay. Phytochemical screenings have done by using usual procedures. Results: an ex-vivo anti-inflammatory test demonstrated that; mean inhibition of protein denaturation 0f 1000/kg of crude methanolic extract of leave of m. Roxburghii was 17.399%. Cardioprotective properties of different extracts of m. Roxburghii was demonstrated which revealed that after treatment of clot with mother methanolic extract, dcm extract (fraction 1), ethyl acetate extract (fraction 2), residual methanolic extract (fraction3) of leaves and methanolic extract of root % clot lysis was 30.56%,27.61%,46.35%,26.02%,49.90 % respectively. Different tests with crude extracts showed that different types of alkaloids, carbohydrates, tannins were present in the leaves of m. Roxburghii. Conclusion: in these investigations demonstrates that different extracts of m. Roxburghii has significant pharmacological activities.

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 5-1)

This article belongs to the Special Issue Herbal Remedies as Alternative to Future Drugs Development and Treatment

DOI 10.11648/j.ajcem.s.2015030501.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Ex-vivo Anti-inflammatory, Cardioprotective Properties, Phytochemicals Investigation, Mean Inhibition of Protein Denaturation

References
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[2] Saha j, sarkar pk, chattopadhyay s. 2011. A survey of ethnomedicinal plants of darjeeling hills for their antimicrobial and antioxidant activities. Ind j nat prod res 2: 479 - 492.
[3] Patil sa, joshi vg. 2011. Evaluation of antibacrerial and wound healing activity of leaves of mussaenda frondosa linn. Int j res pharmac biomed sc 2: 147 - 154.
[4] Das hb, majumdar k, datta bk, ray d. 2009. Ethnobotanical uses of some medicinal plants by tripuri and reang tribes of tripura. Nat prod rad 8: 172 - 180.
[5] Rahman ma. 2010. Indigenous knowledge of herbal medicines in bangladesh and treatment of skin diseases by tribal communities of the hill tracts districts. Bangladesh j bot 39: 169 – 177
[6] Chandra du, ghosh r, chowdhury s, dinda b. 2012. New iridoid from aerial parts of mussaenda roxburghii. Nat prod commun 7: 1 - 2.
[7] Van wagenen bc, larsen r, cardellina jh, ran dazzo d, lidert zc and swithenbank c: ulosantoin (1993) a potent insecticide from the sponge ulosaruetzleri. Journal of organic chemistry 58: 335-337.
[8] Mizushima y and kobayashi m. Interaction of anti-inflammatory drugs with serum preoteins, especially with some biologically active proteins. J of pharma pharmacol. 1968; 20:169-173.
[9] Reyad-ul-ferdous m, alam tt, islam ma, khan mzi, tasnim f, et al (2014) ex-vivo cardioprotective and cytotoxic screening of fruits of parmentiera ereifera seem. Biol med 6: 219. Doi: 10.4172/0974-8369.1000219.
[10] Md. Reyad-ul-ferdous, sayma akhter, md. Zahirul islam khan, md. Eshak khan, md. Atiqul islam, md. Sharif ullah. Ex-vivo anti-inflammatory and antimicrobial activities of the leaves of bauhinia acuminata. American journal of life sciences. Vol. 2, no. 5, 2014, pp. 267-270. Doi: 10.11648/j.ajls.20140205.13.
[11] Daginawala hf, prasad s, kashyap rs, deopujari jy, purohit hj and taori gm (2006) development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thrombosis journal 4: 14.
[12] Kawsar mh, sikder ma, rana ms, nimmi i and rashid ma (2011) studies of thrombolytic and cytotoxic properties of two asteraceous plants of bangladesh. Bangladesh pharmaceutical journal 14: 103-106.
[13] Md. Reyad-ul-ferdous, nasir uddin, d.m. shamim shahjahan, mosharaf hossen, md. Saiful islam arman, ariful islam. Preliminaryin-vitro potential phytochemicals investigation of barks of ravenala madagascariensis sonnerat. Wjpps, 2014; 3(9), 1418-1423.
[14] Egwaikhide, p.a., s.o. okeniyi and c.e. gimba, 2007. Screening for antimicrobial activity and phytochemical constituents of some nigerian medicinal plants. Adv. Biological res., 1: 155-158.
[15] Harborne, j.b. phytochemical methods. A guide to modern techniques of plant analysis. 3rd edn., new york; chapman and hall int ed.1998.
[16] Gani a. Methods of screening, practical phytochemistry,2nd edn, dhaka:chaya prokashoni; 2007.p. 12-17.
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    Farzana Akther Sumi, Biswajit Sikder, Prawej Ansari, Md. Reyad-ul- Ferdous, Anaytulla, et al. (2015). Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook. American Journal of Clinical and Experimental Medicine, 3(5-1), 1-6. https://doi.org/10.11648/j.ajcem.s.2015030501.11

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    ACS Style

    Farzana Akther Sumi; Biswajit Sikder; Prawej Ansari; Md. Reyad-ul- Ferdous; Anaytulla, et al. Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook. Am. J. Clin. Exp. Med. 2015, 3(5-1), 1-6. doi: 10.11648/j.ajcem.s.2015030501.11

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    AMA Style

    Farzana Akther Sumi, Biswajit Sikder, Prawej Ansari, Md. Reyad-ul- Ferdous, Anaytulla, et al. Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook. Am J Clin Exp Med. 2015;3(5-1):1-6. doi: 10.11648/j.ajcem.s.2015030501.11

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  • @article{10.11648/j.ajcem.s.2015030501.11,
      author = {Farzana Akther Sumi and Biswajit Sikder and Prawej Ansari and Md. Reyad-ul- Ferdous and Anaytulla and Mustafe Khalid Mohamed and Md. Mustarek Uddin Meemo},
      title = {Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {5-1},
      pages = {1-6},
      doi = {10.11648/j.ajcem.s.2015030501.11},
      url = {https://doi.org/10.11648/j.ajcem.s.2015030501.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.s.2015030501.11},
      abstract = {Background: mussaenda roxburghii hook. Belongs to family rubiaceae, is a perennial shrub moist area of valley and grows in the foothills. Objective: the present investigation was carried out to phytochemicals and pharmacological activity such anti-inflammatory, cardioprotective properties of methanolic crude extract & its fractions such as dcm extract (fraction 1), ethyl acetate extract (fraction2). Materials and methods: residual methanolic extract (fraction 3) of mussaenda roxburghii leaves which were obtained by solvent-solvent extraction process from crude methanolic extract of mussaenda roxburghii. Anti-inflammatory activity was measured by observing the mean inhibition of protein denaturation. Cardioprotective activity was evaluated by thrombolytic assay. Phytochemical screenings have done by using usual procedures. Results: an ex-vivo anti-inflammatory test demonstrated that; mean inhibition of protein denaturation 0f 1000/kg of crude methanolic extract of leave of m. Roxburghii was 17.399%. Cardioprotective properties of different extracts of m. Roxburghii was demonstrated which revealed that after treatment of clot with mother methanolic extract, dcm extract (fraction 1), ethyl acetate extract (fraction 2), residual methanolic extract (fraction3) of leaves and methanolic extract of root % clot lysis was 30.56%,27.61%,46.35%,26.02%,49.90 % respectively. Different tests with crude extracts showed that different types of alkaloids, carbohydrates, tannins were present in the leaves of m. Roxburghii. Conclusion: in these investigations demonstrates that different extracts of m. Roxburghii has significant pharmacological activities.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook
    AU  - Farzana Akther Sumi
    AU  - Biswajit Sikder
    AU  - Prawej Ansari
    AU  - Md. Reyad-ul- Ferdous
    AU  - Anaytulla
    AU  - Mustafe Khalid Mohamed
    AU  - Md. Mustarek Uddin Meemo
    Y1  - 2015/07/15
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajcem.s.2015030501.11
    DO  - 10.11648/j.ajcem.s.2015030501.11
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.s.2015030501.11
    AB  - Background: mussaenda roxburghii hook. Belongs to family rubiaceae, is a perennial shrub moist area of valley and grows in the foothills. Objective: the present investigation was carried out to phytochemicals and pharmacological activity such anti-inflammatory, cardioprotective properties of methanolic crude extract & its fractions such as dcm extract (fraction 1), ethyl acetate extract (fraction2). Materials and methods: residual methanolic extract (fraction 3) of mussaenda roxburghii leaves which were obtained by solvent-solvent extraction process from crude methanolic extract of mussaenda roxburghii. Anti-inflammatory activity was measured by observing the mean inhibition of protein denaturation. Cardioprotective activity was evaluated by thrombolytic assay. Phytochemical screenings have done by using usual procedures. Results: an ex-vivo anti-inflammatory test demonstrated that; mean inhibition of protein denaturation 0f 1000/kg of crude methanolic extract of leave of m. Roxburghii was 17.399%. Cardioprotective properties of different extracts of m. Roxburghii was demonstrated which revealed that after treatment of clot with mother methanolic extract, dcm extract (fraction 1), ethyl acetate extract (fraction 2), residual methanolic extract (fraction3) of leaves and methanolic extract of root % clot lysis was 30.56%,27.61%,46.35%,26.02%,49.90 % respectively. Different tests with crude extracts showed that different types of alkaloids, carbohydrates, tannins were present in the leaves of m. Roxburghii. Conclusion: in these investigations demonstrates that different extracts of m. Roxburghii has significant pharmacological activities.
    VL  - 3
    IS  - 5-1
    ER  - 

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Author Information
  • Department of Pharmacy, North South University, Dhaka, Bangladesh

  • Department of Pharmacy, North South University, Dhaka, Bangladesh

  • Department of Pharmacy, North South University, Dhaka, Bangladesh

  • Department of Pharmacy, North South University, Dhaka, Bangladesh

  • Department of Pharmacy, North South University, Dhaka, Bangladesh

  • Department of Pharmacy, North South University, Dhaka, Bangladesh

  • Department of Pharmacy, North South University, Dhaka, Bangladesh

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